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	Provedor de dados Anais da ABC (AABC) (2) Autor Wei,Tao (2) Hu,Jinqiang (1) Mao,Duobin (1) Sun,Siwen (1) Xu,Chunping (1) Yang,Kunpeng (1) Zang,Jie (1) Zhao,Aijing (1) Mais... Palavra-chave Acetic acid (1) Bifidobacterium (1) Cigarette smoke condensate (1) DNA nuclease (1) Dimer (1) Exopolysaccharides (1) Interaction (1) Recombinational repair (1) StoNurA (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2018 (1) 2015 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Biochemical characterization of the nuclease StoNurA from the hyperthermophilic archaeon Sulfolobus tokodaii Anais da ABC (AABC) Wei,Tao; Yang,Kunpeng; Zang,Jie; Mao,Duobin. Abstract The DNA nuclease gene ST2109 has been cloned from the hyperthermophilic archaeon Sulfolobus tokodaii and expressed in Escherichia coli. The recombinant protein StoNurA has been purified to homogeneity by affinity chromatography and gel filtration chromatography. Biochemical analyses demonstrated that StoNurA exhibited DNA binding and 5’–3’ exonuclease activities towards ssDNA and dsDNA. The temperature and pH optima of StoNurA were determined to be 65 °C and 8.0, respectively. The activity of StoNurA was found to be dependent of Mn2+, and its half-life of heat inactivation at 100 °C was 5 min. Gel filtration chromatography revealed that StoNurA could form dimers in solution. Pull-down assays also showed that StoNurA physically interacted with a... Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA nuclease; StoNurA; Dimer; Interaction; Recombinational repair. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000602731 Effects of cigarette smoke condensate on the production and characterization of exopolysaccharides by Bifidobacterium Anais da ABC (AABC) Hu,Jinqiang; Wei,Tao; Sun,Siwen; Zhao,Aijing; Xu,Chunping. The aim of the study was to investigate the effect of cigarette smoke on the production and characterization of exopolysaccharides (EPSs) produced by Bifidobacterium. Cigarettes of Shanhua brand (nicotine: 1.1 mg, tar: 11 mg) were utilized to prepare a cigarette smoke condensate (CSC). The standard strain of Bifidobacterium animalis was cultured in MRS media under anaerobic addition of CSC. The results showed that CSC significantly decreased the growth of B. animalis as well as EPSs and acetic acid production. Furthermore, two EPSs fractions (Fr-I and Fr-II) were isolated and purified for chemical and molecular determination. By comparison with control, CSC was found to be of great impact on EPSs carbohydrate composition. The molecular weight mass of Fr-I... Tipo: Info:eu-repo/semantics/article Palavras-chave: Exopolysaccharides; Cigarette smoke condensate; Bifidobacterium; Acetic acid. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000200997 Registros recuperados: 2 Primeira ... 1 ... Última

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